Science & technology

Nirenberg and Matthaei crack the first word of the genetic code

May 1961

In May 1961 at the National Institutes of Health in Bethesda, the biochemist Marshall Nirenberg and the postdoctoral researcher Heinrich Matthaei added a synthetic RNA made only of uracil (poly-U) to a cell-free extract of bacteria and found that it produced a protein made only of the amino acid phenylalanine. So the three-letter word UUU stands for phenylalanine, the first entry in the genetic code. Nirenberg announced the result at a conference in Moscow in August 1961. Within five years, Nirenberg, Har Gobind Khorana and others had worked out all 64 codons. Nirenberg, Khorana and Robert Holley shared the 1968 Nobel Prize.

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